Mutational spectrometry without phenotypic selection: human mitochondrial DNA
نویسندگان
چکیده
منابع مشابه
Mutational spectrometry without phenotypic selection: human mitochondrial DNA.
By first separating mutant from nonmutant DNA sequences on the basis of their melting temperatures and then increasing the number of copies by high-fidelity DNA amplification, we have developed a method that allows observation of point mutations in biological samples at fractions at or above 10-6. Using this method, we have observed the hotspot point mutations that lie in 100 base pairs of the ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1997
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/25.4.685